![]() ![]() Thus, the coproduced enzyme mixture from a bacterium with halotolerance is plausible in pretreated lignocellulose degradation. ![]() After 24 h of enzymatic treatment, the saccharification rates of the coproduced enzyme mixture were 38.96% on rice bran, 49.85% on wheat bran, 61.2% on cassava bagasse and 57.82% on corn cob. There were 3.29, 1.81 and 2.08 fold increase in enzyme activity with respect to agarase, amylase and xylanase after optimization against basal medium. The preponderant variables (pH, temperature, lactose) and their interaction effect on enzyme production were studied by Plackett-Burman design and Response Surface Methodology (RSM). The optimum production conditions clinched by classical optimization were: pH 8 1.5% inoculum 24 h incubation, 40 ☌ 8% NaCl (sodium chloride) 1% lactose and NaNO 3 (sodium nitrate). Bacterial strain, Halomonas meridiana VITSVRP14, isolated from seaweed was labored to produce amylase, agarase and xylanase conjointly using submerged fermentation. Coproduction of multienzymes from single potential microbe has captivated contemplation in industries. ![]()
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